Anlysis in Biochmeistry Lab Report Example | Topics and Well Written Essays - 1000 words

Anlysis in Biochmeistry - Lab Report Example The risk can be minimsed by using reliable controls for background fluorescence check as is used in the current study. The average percentage inhibition of the cytochrome P450 3A4 by ketaconazole was determined using a fluorescence based enzyme assay. The readings were obtained as per procedure and is recorded and calculated in Tables 1, 2, 3 and 4. Ketaconazole samples in varying concentrations showed lower fluorescence than the sample without the test compound indicating the inhibitory activity of the drug on the enzyme cytochrome P450. Saple with 10 M concentration showed the least fluorescence and therefore the highest % inhibition of 95.274.7 and even a lower concentration of 0.1 M also shows a percentage inhibition of about 75.616.12. The fluorescence reading shows the inhibition of the enzyme to be directly proportional to the concentration of the test compound ketaconazole. The present study a fluorescence based assay to illustrate and evaluate the interaction of ketaconazole with the liver cytochrome P450 A4 using microsomes prepared from insect cells that express both human P450 isozyme and rabbit NADPH-P450 reductase and a substrate th

Sound Waves Lab Report Example | Topics and Well Written Essays - 250 words

Sound Waves - Lab Report Example Fourier Transform essentially incorporates a time series of pressure intensity in the determination of the perfect pitched frequencies summation. It also incorporates the level of the amplitude to recreate the original sound. Series of data aids in telling the amplitude of the sound as a function of time to a corresponding series data that determines the amplitude of the frequencies for recreation of the sound. Based on the graphs collected the ambiguity within the time durations and frequencies measured for diverse sound waves emanated from the errors in the measurement Logger Pro. The percentage error amidst the tuning fork frequency and corresponding measured frequencies was less that 1%, which is relatively safe. The percentage error amidst the printed frequency and the measured frequency was 0.58% as depicted by the sine curve. Undertaking the curve fit is relatively more precise measurement method regarding finding the frequency since it is not subjective to many of individual’s errors. The concept of Fourier Transforms is fundamental in the examination of the sound waves. Moreover, it integrates a time series of pressure intensity in the resolving the perfect pitched frequencies summation. Series of data display amplitude of the sound as a function of time to a corresponding series data that determines the amplitude of the frequencies for recreation of the sound. There was certain error in the lab experiment that mainly occurs due to the individual’s point of view of the underlying maximum intensity, within is not fundamental to other